The Alphabet (1968) combines animation and live-action and goes for four minutes. It has a simple narrative structure relating a symbolically rendered expression of a fear of learning.
The idea for The Alphabet came from Lynch’s wife, Peggy Lentz, a painter whose niece, according to Lynch in Chris Rodley’s Lynch on Lynch book, “was having a bad dream one night and was saying the alphabet in her sleep in a tormented way. So that’s sort of what started The Alphabet going.” Based on the merits of this short film, Lynch was awarded an American Film Institute production grant and became a minor celebrity. – Wikipedia